Jonathan Stefely

Postdoctoral Research Associate




(651) 717-5099


[javascript protected email address]

I study metabolism through systems biology and chemical biology.

Areas of Expertise

  • Biochemistry
  • Chemical biology
  • Organic chemistry
  • Yeast biology
  • Metabolism
  • Enzymology
  • Biosynthesis
  • Systems biology


Ph.D. Biochemistry, 2015, University of Wisconsin-Madison

B.S. Chemistry, 2009, University of Notre Dame


NIH Predoctoral Fellow (Ruth L Kirchstein NRSA, F30) (2012-2015)

McKnight Prize in Undergraduate Chemistry (2008)

Eagle Scout (2003)

Selected Publications

  • Stefely, J.A.; Reidenbach, A.G.; Ulbrich, A.; Oruganty, K.; Floyd, B.J.; Jochem, A.; Saunders, J.M.; Johnson, I.E.; Minogue, C.E.; Wrobel, R.L.; Barber, G.E.; Lee, D.; Li, S.; Kannan, N.; Coon, J.J.; Bingman, C.A.; Pagliarini, D.J. Mitochondrial ADCK3 employs an atypical protein kinase-like fold to enable coenzyme Q biosynthesis. Mol. Cell 2015, 57, 83– 94
  • Lohman, D.C.; Forouhar, F.; Beebe, E.T.; Stefely, M.S.; Minogue, C.E.; Ulbrich, A.; Stefely, J.A.; Sukumar, S.; Luna-Sánchez, M.; Jochem, A.; Lew, S.; Seetharaman, J.; Xiao, R.; Wang, H.; Westphall, M.S.; Wrobel, R.L.; Everett, J.K.; Mitchell, J.C.; López, L.C.; Coon, J.J.; Tong, L.; Pagliarini, D.J. Mitochondrial COQ9 is a lipid-binding protein that associates with COQ7 to enable coenzyme Q biosynthesis. Proc. Natl. Acad. Sci. U S A. 2014, 111, E4697– 705.
  • Khadria, A.S.; Mueller, B.K.; Stefely, J.A.; Tan, C.H.; Pagliarini, D.J.; Senes, A. A Gly- zipper motif mediates homodimerization of the transmembrane domain of the mitochondrial kinase ADCK3. J. Am. Chem. Soc. 2014, 136, 14068–77.
  • Hebert, A.S.; Merrill, A.E.; Stefely, J.A.; Bailey, D.J.; Wenger, C.D.; Westphall, M.S.; Pagliarini, D.J.; Coon, J.J. Amine-reactive Neutron-encoded Labels for Highly Plexed Proteomic Quantitation. Mol. Cell. Proteomics 2013, 12, 3360–3369.
  • Stefely, J.A.; Palchaudhuri, R.; Miller, P.A.; Peterson, R.J.; Moraski, G.C.; Hergenrother, P.J.; Miller, M.J. N-((1-Benzyl-1H-1,2,3-triazol-4-yl)methyl)arylamide as a New Scaffold that Provides Rapid Access to Antimicrotubule Agents: Synthesis and Evaluation of Antiproliferative Activity Against Select Cancer Cell Lines. J. Med. Chem. 2010, 53, 3389- 3395.