The overall goal of my lab is the systematic study of developmental processes in living organisms by noninvasive biomedical imaging techniques such as optical microscopy. Of primary interest is the investigation of organogenesis in zebrafish with special emphasis on the function and morphogenesis of the cardiovascular system and the endoderm. We develop novel quantitative microscopy tools and experimental strategies to understand and describe tissue dynamics on a cellular level. High-speed fluorescence microscopy is the primary tool to capture the dynamics of a heartbeat and the fate of single cells during organogenesis.
In our interdisciplinary lab we address all experimental steps from innovative transgenic lines and microscope development to systematic image processing. The biologists in the lab have the opportunity to use cutting edge microscopy to perform experiments that are impossible with off-the-shelf instruments. At the same time the physicists in the lab can build novel microscopes that are immediately applied to address exciting biological questions.
Areas of Expertise
- Selective plane illumination microscopy (SPIM)
- High-speed fluorescence microscopy
- Zebrafish, with special focus on the cardiovascular system and endoderm
Director of Morgridge Medical Engineering at Morgridge Institute for Research, Madison
Professor of Biomedical Engineering at University of Wisconsin, Madison
Research Group Leader at the Max Planck Institute of Molecular Cell Biology and Genetics
Postdoctoral work at the University of California, San Francisco
Postdoctoral work at the EMBL-Heidelberg
PhD work at the EMBL-Heidelberg, PhD in Physics, University of Freiburg
- Rory M Power, Jan Huisken, A guide to light-sheet fluorescence microscopy for multiscale imaging., Nat Methods, 14(4) 360-373 (2017).
- Wiebke Jahr, Benjamin Schmid, Christopher Schmied, Florian Fahrbach, Jan Huisken Hyperspectral light sheet microscopy. Nat Commun, 6 Art. No. 7990 (2015).
- Nico Scherf, Jan Huisken The smart and gentle microscope. Nat Biotechnol, 33(8) 815-818 (2015).
- Andrea Bassi, Benjamin Schmid, Jan Huisken Optical tomography complements light sheet microscopy for in toto imaging of zebrafish development. Development, 142(5) 1016-1020 (2015).
- Michaela Mickoleit, Benjamin Schmid, Michael Weber, Florian Fahrbach, Sonja Hombach, Sven Reischauer, Jan Huisken, High-resolution reconstruction of the beating zebrafish heart., Nat Methods, 11(9) 919-922 (2014).
- Benjamin Schmid, Gopi Shah, Nico Scherf, Michael Weber, Konstantin Thierbach, Claudia Campos, Ingo Roeder, Pia Aanstad, Jan Huisken, High-speed panoramic light-sheet microscopy reveals global endodermal cell dynamics., Nat Commun, 4 Art. No. 2207 (2013).
- Anna Kaufmann, Michaela Mickoleit, Michael Weber, Jan Huisken, Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope., Development, 139(17) 3242-3247 (2012).
- Aristides B. Arrenberg, Didier Y.R. Stainier, Herwig Baier, Jan Huisken, Optogenetic control of cardiac function., Science, 330(6006) 971-974 (2010).
- Shane P Herbert, Jan Huisken, Tyson N Kim, Morri E Feldman, Benjamin T Houseman, Rong A Wang, Kevan M Shokat, Didier Y.R. Stainier, Arterial-venous segregation by selective cell sprouting: an alternative mode of blood vessel formation., Science, 326(5950) 294-298 (2009).
- Jan Huisken, Didier Y.R. Stainier, Selective plane illumination microscopy techniques in developmental biology., Development, 136(12) 1963-1975 (2009).
- Jan Huisken, Didier Y.R. Stainier, Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM)., Opt Lett, 32(17) 2608-2610 (2007).
- Jan Huisken, Jim Swoger, Filippo Del Bene, Jochen Wittbrodt, Ernst H K Stelzer, Optical sectioning deep inside live embryos by selective plane illumination microscopy., Science, 305(5686) 1007-1009 (2004).