Jan Huisken

Affiliate, Morgridge Biomedical Imaging

Area:

• Biomedical Imaging

Phone:

(608) 316-4417

Email:

[javascript protected email address]

Huisken has been awarded a prestigious Alexander von Humboldt Professorship from Germany, and in October 2021 accepted a research position at the University of Göttingen. Huisken remains an affiliate of the Morgridge Institute.

The overall goal of my lab is the systematic study of developmental processes in living organisms by noninvasive biomedical imaging techniques such as optical microscopy. Of primary interest is the investigation of organogenesis in zebrafish with special emphasis on the function and morphogenesis of the cardiovascular system and the endoderm. We develop novel quantitative microscopy tools and experimental strategies to understand and describe tissue dynamics on a cellular level. High-speed fluorescence microscopy is the primary tool to capture the dynamics of a heartbeat and the fate of single cells during organogenesis.

In our interdisciplinary lab we address all experimental steps from innovative transgenic lines and microscope development to systematic image processing. The biologists in the lab have the opportunity to use cutting edge microscopy to perform experiments that are impossible with off-the-shelf instruments. At the same time the physicists in the lab can build novel microscopes that are immediately applied to address exciting biological questions.

Areas of Expertise

• Selective plane illumination microscopy (SPIM)
• High-speed fluorescence microscopy
• Organogenesis
• Zebrafish, with special focus on the cardiovascular system and endoderm

Since 2016

Director of Morgridge Medical Engineering at Morgridge Institute for Research, Madison
Visiting Professor, Department of Integrative Biology

2010-2017

Research Group Leader at the Max Planck Institute of Molecular Cell Biology and Genetics

2005-2009

Postdoctoral work at the University of California, San Francisco

2004-2005

Postdoctoral work at the EMBL-Heidelberg

2000-2004

PhD work at the EMBL-Heidelberg, PhD in Physics, University of Freiburg

Selected Publications

• Image quality guided smart rotation improves coverage in microscopy
  J. He, J. Huisken
  Nature Communications 11, 150 (2020)
• Multi-scale imaging and analysis identify pan-embryo cell dynamics of germlayer formation in zebrafish
  G. Shah, K. Thierbach, B. Schmid, J. Waschke, A. Reade, M. Hlawitschka, I. Roeder, N. Scherf, J. Huisken
  Nature Communications 10, 5753 (2019)
• Putting Advanced Microscopy in the Hands of Biologists
  R.M. Power, J. Huisken
  Nature Methods 16, 1069 (2019)
• Multi-sample SPIM image acquisition, processing and analysis of vascular growth in zebrafish
  S. Daetwyler, U. Günther, C.D. Modes, K. Harrington, J. Huisken
  Development 146, dev173757 (2019)
• Dynamic, non-contact 3D sample rotation for microscopy
  F. Berndt, G. Shah, J. Brugues, J. Huisken
  Nature Communications 9, 5025 (2018)
• Cell-accurate optical mapping across the entire developing heart
  Michael Weber, Nico Scherf, Alexander M Meyer, Daniela Panáková, Peter Kohl, Jan Huisken
  eLife 6 (2017), doi:10.7554/eLife.28307
• A guide to light-sheet fluorescence microscopy for multiscale imaging
  Rory M Power, Jan Huisken
  Nat Methods, 14(4) 360-373 (2017), doi:10.1038/nmeth.4224
• Hyperspectral light sheet microscopy
  Wiebke Jahr, Benjamin Schmid, Christopher Schmied, Florian Fahrbach, Jan Huisken
  Nat Commun, 6 Art. No. 7990 (2015), doi:10.1038/ncomms899
• The smart and gentle microscope
  Nico Scherf, Jan Huisken
  Nat Biotechnol, 33(8) 815-818 (2015), doi:10.1038/nbt.3310
• Optical tomography complements light sheet microscopy for in toto imaging of zebrafish development
  Andrea Bassi, Benjamin Schmid, Jan Huisken
  Development, 142(5) 1016-1020 (2015), doi: 10.1242/dev.116970
• High-resolution reconstruction of the beating zebrafish heart
  Michaela Mickoleit, Benjamin Schmid, Michael Weber, Florian Fahrbach, Sonja Hombach, Sven Reischauer, Jan Huisken
  Nat Methods, 11(9) 919-922 (2014) , doi:10.1038/nmeth.3037
• High-speed panoramic light-sheet microscopy reveals global endodermal cell dynamics
  Benjamin Schmid, Gopi Shah, Nico Scherf, Michael Weber, Konstantin Thierbach, Claudia Campos, Ingo Roeder, Pia Aanstad, Jan Huisken
  Nat Commun, 4 Art. No. 2207 (2013), doi:10.1038/ncomms3207
• Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope
  Anna Kaufmann, Michaela Mickoleit, Michael Weber, Jan Huisken
  Development, 139(17) 3242-3247 (2012), doi: 10.1242/dev.082586
• Optogenetic control of cardiac function
  Aristides B. Arrenberg, Didier Y.R. Stainier, Herwig Baier, Jan Huisken
  Science, 330(6006) 971-974 (2010), DOI: 10.1126/science.1195929
• Arterial-venous segregation by selective cell sprouting: an alternative mode of blood vessel formation
  Shane P Herbert, Jan Huisken, Tyson N Kim, Morri E Feldman, Benjamin T Houseman, Rong A Wang, Kevan M Shokat, Didier Y.R. Stainier
  Science, 326(5950) 294-298 (2009), doi: 10.1126/science.1178577
• Selective plane illumination microscopy techniques in developmental biology
  Jan Huisken, Didier Y.R. Stainier
  Development, 136(12) 1963-1975 (2009), doi: 10.1242/dev.022426
• Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM)
  Jan Huisken, Didier Y.R. Stainier
  Opt Lett, 32(17) 2608-2610 (2007), https://doi.org/10.1364/OL.32.002608
• Optical sectioning deep inside live embryos by selective plane illumination microscopy
  Jan Huisken, Jim Swoger, Filippo Del Bene, Jochen Wittbrodt, Ernst H K Stelzer
  Science, 305(5686) 1007-1009 (2004), DOI: 10.1126/science.1100035