Model is of a prevacuolar compartment of a high-pressure frozen/freeze substituted maize aleurone cell that was imaged by dual electron tomography in a Tecnai F30 transmission electron microscope (Reyes, F. C., Chung, T., Holding, D., Jung, R., Vierstra, R. & Otegui, M. S. (2011) Delivery of prolamins to the protein storage vacuole in maize aleurone cells. Plant Cell 23, 769-784.) A physical model (left) was created by 3D printing a mold of the extents of the model and filling it with acrylic resin to form a completed part. Individual components of the model and filling it with acrylic resin to form a completed part. Individual components of the model were printed with different color plastics and placed inside the mold at approximate positions while it was being filled with resin.