Dave Pagliarini

Director, Metabolism Research Group

Area:

Metabolism

Phone:

(608) 316-4664

Email:

[javascript protected email address]

Laboratory website: www.pagliarinilab.org

I lead the Metabolism research theme at Morgridge. In this role, I am helping to build a campus-wide metabolism initiative by recruiting talented and collaborative new investigators, assisting with the development of new leading-edge metabolism-related facilities, and promoting a more cohesive metabolism research culture in Madison. I am also an Associate Professor of Biochemistry at UW-Madison. My laboratory is an interdisciplinary team of scientists driven to understand the biochemical underpinnings of mitochondrial dysfunction in human diseases. We integrate large-scale methodologies with traditional biochemistry to investigate the modulation, adaptation and basic metabolic function of mitochondria.

Education

Postdoctoral fellowship, 2009, Harvard Medical School

Ph.D., 2005, University of California, San Diego

M.S., 2002, University of Michigan

B.S., 1999, University of Notre Dame

Selected Honors & Awards

  • 2016 Pound Research Award, UW–Madison
  • 2016 Presidential Early Career Award for Scientists and Engineers (PECASE)
  • 2015 Vilas Associates Award, UW–Madison
  • 2012 Shaw Scientist Award, The Greater Milwaukee Foundation
  • 2011 Searle Scholar Award, Kinship Foundation
  • 2011 Ellison Medical Foundation New Scholar Award in Aging (selected, but ineligible to accept)
  • 2011 Glenn Award, Glenn Foundation for Medical Research

Selected Publications

  • Guo X*, Niemi NM*, Hutchins PD, Condon SGF, Jochem A, Ulbrich A, Higbee AJ, Russell JD, Senes A, Coon JJ, and Pagliarini DJ, Ptc7p dephosphorylates select mitochondrial proteins to enhance metabolic function, Cell Reports, 2017 18: 1–7
  • Stefely JA*, Kwiecien NW*, Freiberger E, Richards AL, Jochem A, Rush MJP, Ulbrich A, Robinson KP, Hutchins PD, Veling MT, Guo X, Kemmerer ZA, Connors KJ, Trujillo EA, Sokol J, Westphall MW, Hebert AS, Pagliarini DJ, and Coon JJ†, Mitochondrial protein functions elucidated by global mass spectrometry profiling, Nature Biotechnology, 2016 34(11):1191-1197. 
  • Rensvold JW, Krautkramer KA, Dowell JA, Denu JM, and Pagliarini DJ, Iron Deprivation Induces Transcriptional Regulation of Mitochondrial Biogenesis, Journal of Biological Chemistry, 2016 291(40):20827-20837.
  • Floyd BJ*, Minogue CE*, Wilkerson EM*, Veling MT*, Xia C, Beebe ET, Wrobel, RL, Cho H, Kremer LS, Alston CL, Gromek KA, Dolan BK, Ulbrich A, Stefely JA, Bohl SL, Werner KM, Jochem A, Westphall MS, Rensvold JW, Taylor RW, Prokisch H, Kim JJ, Coon JJ and Pagliarini DJ, Mitochondrial protein interaction mapping identifies new regulators of respiratory chain function, Molecular Cell, 2016 63(4): 621-32. 
  • Stefely JA*, Licitra F*, Laredj L, Reidenbach AG, Kemmerer AZ, Grangeray A, Jaeg-Ehret T, Minogue CE, Ulbrich A, Hutchins PD, Wilkerson EM, Ruan Z, Aydin D, Hebert AS, Guo X, Freiberger EC, Reutenauer L, Jochem A, Chergova M, Johnson IE,  Lohman DC, Rush MJP, Kwiecien NW, Singh PK, Schlagowski AI, Floyd BJ, Forsman U, Sindelar P, Westphall MS, Pierell F, Zoll J, Dal Peraro M, Kannan N, Bingman CA Coon JJ, Isope P, Puccio H†, and Pagliarini DJ, Cerebellar Ataxia and Coenzyme Q  Deficiency Through Loss of Unorthodox Kinase Activity,Molecular Cell, 2016 63(4):608-20.
  • Tagliabracci VS*, Wiley SE*, Guo X, Kinch LN, Durrant E, Wen J, Xiao J, Cui J, Engel JL, Coon JJ, Grishin N, Pinna LA, Pagliarini DJ and Dixon JE, A single kinase generates the majority of the secreted phosphoproteome, Cell, 2015, 18;161(7):16-19-32.
  • Overmyer KA, Evans CR, Qi NR, Minogue CV, Chermside-Scaboo CJ, Kock LG, Britton SL, Pagliarini DJ, Coon JJ and Burant CF, Maximal oxidative capacity during exercise is associated with skeletal muscle fuel selection and dynamic changes in mitochondrial protein acetylation, Cell Metabolism, 2015, 3;21(3):468-78.
  • Stefely JA*, Reidenbach AG*, Ulbrich A, Oruganty K, Floyd BJ, Jochem A, Saunders JM, Johnson IE, Minogue CE, Wrobel RL, Barber GE, Lee D, Li S, Kannan N, Coon JJ, Bingman CA and Pagliarini DJ, Mitochondrial ADCK3 employs an atypical protein kinase-like fold to enable coenzyme Q biosynthesis, Molecular Cell, 2015, 57: 83-94.
  • Lohman DC*, ForouharF*, Beebe ET, Stefely MS, Minogue CE, Ulbrich A, Stefely JA, Sukumar S, Luna-Sanchez M, Lew S, Seetharaman J, Xiao R, Wang H, Wrobel RL, Everett JK, Mitchell JC, Lopez LC, Coon JJ, Tong L† and Pagliarini DJ, Mitochondrial COQ9 is a lipid binding protein that associates with COQ7 to enable coenzyme Q biosynthesis, Proceedings of the National Academy of Sciences, 2014, 111(44):E4697-705 (Direct submission)
  • Khadria AS, Mueller BK, Stefely JA, Tan CH, Pagliarini DJ and Senes A†, A Gly-zipper motif mediates homo-dimerization of the transmembrane domain of the mitochondrial kinase ADCK3, Journal of the American Chemical Society, 2014,111(44):E4697-705. 
  • Pagliarini DJand Rutter J†, Hallmarks of a new era in mitochondrial biochemistry. Genes & Development, 2013, 27(24): 2615-27
  • Still AJ*, Floyd BJ*, Hebert AS*, Bingman CA, Gunderson DR, Dolan BK, Carson JJ, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ and Pagliarini DJ, Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation. Journal of Biological Chemistry,2013, 288(36):26209-19.
  • Rensvold JW, Ong SE, Jeevananthan A, Carr SA, Mootha VK and Pagliarini DJ, Complementary RNA and Protein Profiling Identifies Iron as a Key Regulator of Mitochondrial Biogenesis, Cell Reports, 2013, 3(1): 237-245 
  • Grimsrud PA*, Carson JJ*, Hebert A, Hubler SL, Niemi NM, Bailey DJ, Jochem A, Stapleton DS, Keller MP, Westphall MS, Yandell BS, Attie AD, Coon JJ, and Pagliarini DJ, A quantitative map of the mitochondrial phosphoproteome reveals post-translational control of ketogenesis, Cell Metabolism, 2012, 16(5):672-83.
  • Calvo SE*, Pagliarini DJ* & Mootha VK, Upstream open reading frames cause widespread reduction of protein expression and are polymorphic among humans, Proceedings of the National Academy of Sciences, 2009,106, 7507-12 (Track II direct submission). PMCID: PMC2669787 
  • Sugiana C*, Pagliarini DJ*, McKenzie M, Kirby DM, Salemi R, Dahl HHM, Hutchison WM, Vascotto KA, Smith SM, Christodoulou J, Calvo SE, Mootha VK, Ryan MT and Thorburn DR, Mutation of C20orf7 disrupts Complex I assembly and causes lethal infantile mitochondrial disease, American Journal of Human Genetics 2008:83(4): p.468-478. PMCID: PMC2561934
  • Pagliarini DJ*, Calvo SE*, Chang B, Sheth SA, Vafai SB, Ong SE, Walford GA, Sugiana C, Boneh A, Chen WK, Hill DE, Vidal M, Evans JG, Thorburn DR, Carr SA, and Mootha VK, A mitochondrial protein compendium elucidates complex I disease biology, Cell, 2008, 134(1): 112-123. PMCID: PMC2778844 
  • Pagliarini DJ, Wiley SE, Kimple ME, Dixon JR, Kelly P, Casey PJ, and Dixon JE, Involvement of a Mitochondrial Phosphatase in the Regulation of ATP Production and Insulin Secretion in Pancreatic b Cells, Molecular Cell, 2005, 19(2): p.197-207