Publications

2019

• Quantitative Spatial Analysis of Metabolic Heterogeneity Across in vivo and in vitro Tumor Models
  Heaster TM, Landman BA, Skala MC
  Front. Oncol., 01 November 2019. doi: fonc.2019.01144
• Classifying T cell activity in autofluorescence intensity images with convolutional neural networks
  Wang ZJ, Walsh AJ, Skala MC, Gitter A
  J Biophotonics. 2019 Oct 29. doi: 10.1002/jbio.201960050.
• Sample Preparation Strategies for High-Throughput Mass Spectrometry Imaging of Primary Tumor Organoids
  Johnson J, Sharick JT, Skala MC, Li L
  Journal of mass spectrometry: JMS, 2019 Oct 29. doi: 10.1002/jms.4452
• Real-time optical redox imaging of cartilage metabolic response to mechanical loading
  Walsh SK, Skala MC, Henak CR
  Osteoarthritis Cartilage. 2019 Sep 9. pii: S1063-4584(19)31190-2. doi: 10.1016/j.joca.2019.08.004.
• Tumor-on-a-chip: a microfluidic model to study cell response to environmental gradients
  Ayuso JM, Virumbrales-Munoz M, McMinn PH, Rehman S, Gomez I, Karim MR, Trusttchel R, Wisinski KB, Beebe DJ, Skala MC
  Lab Chip. 2019 Sep 11. doi: 10.1039/c9lc00270g.
• Effects of culture method on response to EGFR therapy in head and neck squamous cell carcinoma cells
  Ayuso JM, Vitek R, Swick AD, Skala MC, Wisinski KB, Kimple RJ, Lambert PF, Beebe DJ
  Sci Rep. 2019 Aug 28;9(1):12480. doi: 10.1038/s41598-019-48764-3.
• Patient-Derived Cancer Organoid Cultures to Predict Sensitivity to Chemotherapy and Radiation
  Pasch CA, Favreau PF, Yueh AE, Babiarz CP, Gillette AA, Sharick JT, Karim MR, Nickel KP, DeZeeuw AK, Sprackling CM, Emmerich PB, DeStefanis RA, Pitera RT, Payne SN, Korkos DP, Clipson L, Walsh CM, Miller D, Carchman EH, Burkard ME, Lemmon KK, Matkowskyj KA, Newton MA, Ong IM, Bassetti MF, Kimple RJ, Skala MC, Deming DA
  Clin Cancer Res. 2019 Jun 7. doi: 10.1158/1078-0432.CCR-18-3590.
• Cellular Metabolic Heterogeneity In Vivo Is Recapitulated in Tumor Organoids
  Sharick JT, Jeffery JJ. Karim MR, Walsh CM, Esbona K, Cook RS, Skala MC
  Neoplasia 21:6, June 2019, Pg 615-626. doi: 10.1016/j.neo.2019.04.004
• Imaging intratumoral metabolic heterogeneity
  Walsh AJ, Sharick JT, Skala MC
  Nature BME: News & Views. 3, 333–334 (2019). doi: 10.1038/s41551-019-0398-z
• Redox imaging and optical coherence tomography of the respiratory ciliated epithelium
  Gil DA, Sharick JT, Mancha S, Gamm UA, Choma MA, Skala MC
  J Biomed Opt. 2019 Jan 30. doi: 10.1117/1.JBO.24.1.010501.

2018

• Reproducibility and staging of 3D human retinal organoids across multiple pluripotent stem cell lines
  Capowski EE, Samimi K, Mayerl SJ, Phillips MJ, Pinilla I, Howden SE, Saha J, Jansen AD, Edwards KL, Jager LD, Barlow K, Valiauga R, Erlichman Z, Hagstrom A, Sinha D, Sluch VM, Chamling X, Zack DJ, Skala MC, Gamm DM
  Development. 2018 Dec 19. doi: 10.1242/dev.171686.
• Quantitative Label-Free Imaging of 3D Vascular Networks Self-Assembled in Synthetic Hydrogels
  Kaushik G, Gil DA, Torr E, Berge ES, Soref C, Uhl P, Fontana G, Antosiewicz-Bourget J, Edington C, Schwartz MP, Griffith LG, Thomson JA, Skala MC, Daly WT, Murphy WL
  Adv Healthc Mater. 2018 Dec 19 doi: 10.1002/adhm.201801186.
• Evaluating natural killer cell cytotoxicity against solid tumors using a microfluidic model
  Ayuso JM, Truttschel R, Gong MM, Humayun M, Virumbrales-Munoz M, Vitek R, Felder M, Gillies SD, Sondel P, Wisinski KB, Patankar M, Beebe DJ, Skala MC
  OncoImmunology (2018) DOI: 10.1080/2162402X.2018.1553477
• Imaging retinal melanin: a review of current technologies
  Lapierre-Landry M, Carroll J, Skala MC
  J Biol Eng. (2018) Dec 4;12:29. doi: 10.1186/s13036-018-0124-5.
• MTORC1/2 inhibition as a therapeutic strategy for PIK3CA mutant cancers
  Fricke SL, Payne SN, Favreau PF, Kratz JD, Pasch CA, Foley TM, Yueh AE, Van De Hey DR, Depke MG, Korkos DP, Sha GC, DeStefanis RA, Clipson L, Burkard ME, Lemmon KK, Parsons BM, Kenny PA, Matkowskyj KA, Newton MA, Skala MC, Deming DA
  Mol Cancer Ther. (2018) Nov 13. doi: 10.1158/1535-7163.MCT-18-0510.
• BET inhibitors reduce cell size and induce reversible cell cycle arrest in AML
  Zhang S, Zhao Y, Heaster TM, Fischer MA, Stengel KR, Zhou X, Ramsey H, Zhou MM, Savona MR, Skala MC, Hiebert SW
  J Cell Biochem. (2018) Nov 11. doi: 10.1002/jcb.28005.
• Organotypic microfluidic breast cancer model reveals starvation-induced spatial-temporal metabolic adaptations
  Ayuso JM, Gillette A, Lugo-Cintrón K, Acevedo-Acevedod S, Gomez I, Morgan M, Heaster T, Wisinski KB, Palecek SP, Skala MC, Beebe DJ
  EBioMedicine (2018) doi: 10.1016/j.ebiom.2018.10.046
• Imaging Melanin Distribution in the Zebrafish Retina Using Photothermal Optical Coherence Tomography
  Lapierre-Landry M, Huckenpahler AL, Link BA, Collery RF, Carroll J, Skala MC
  Transl Vis Sci Technol. 2018 Sep 4;7(5):4. doi: 10.1167/tvst.7.5.4.
• Photothermal optical coherence tomography of indocyanine green in ex vivo eyes
  Lapierre-Landry M, Connor TB, Carroll J, Tao YK, Skala MC
  Opt Lett. 2018 Vol. 43, Issue 11, pp. 2470-2473. doi: 10.1364/OL.43.002470.
• Protein-bound NAD(P)H Lifetime is Sensitive to Multiple Fates of Glucose Carbon
  Sharick JT, Favreau PF, Gillette AA, Sdao SM, Merrins MJ, Skala MC
  Scientific Reports (2018) Vol. 8, Article number: 5456. doi:10.1038/s41598-018-23691-x
• Quantifying optical properties with visible and near-infrared optical coherence tomography to visualize esophageal microwave ablation zones
  Niemeier RC, Etoz S, Gil DA, Skala MC, Brace CL, and Rogers JD. Biomed
  Opt. Express. 2018 9(4) 1648-1663. doi: 10.1364/BOE.9.001648
• Oxidative stress via inhibition of the mitochondrial electron transport and Nrf-2-mediated anti-oxidative response regulate the cytotoxic activity of plumbagin
  Kapur A, Beres T, Rathi K, Nayak AP, Czarnecki A, Felder M, Gillette A, Ericksen SS, Sampene E, Skala MC, Barroilhet L, Patankar MS
  Sci Rep. 2018 Jan 18;8(1):1073. doi: 10.1038/s41598-018-19261-w.
• Mutant KRAS Exosomes Alter the Metabolic State of Recipient Colonic Epithelial Cells
  Zhang Q, Jeppesen DK, Higginbotham JN, Demory Beckler M, Poulin EJ, Walsh AJ, Skala MC, McKinley ET, Manning HC, Hight MR, Schulte ML, Watt KR, Ayers GD, Wolf MM, Andrejeva G, Rathmell JC, Franklin JL, Coffey RJ.
  Cell Mol Gastroenterol Hepatol. 2018 Jan 31;5(4):627-629.e6. doi: 10.1016/j.jcmgh.2018.01.013
• Pharmacological blockade of ASCT2-dependent glutamine transport leads to antitumor efficacy in preclinical models
  Schulte ML, Fu A, Zhao P, Li J, Geng L, Smith ST, Kondo J, Coffey RJ, Johnson MO, Rathmell JC, Sharick JT, Skala MC, Smith JA, Berlin J, Washington MK, Nickels ML, Manning HC
  Nat Med. 2018 Jan 15. doi: 10.1038/nm.4464.

2017

• Drug-Free ROS Sponge Polymeric Microspheres Reduce Tissue Damage from Ischemic and Mechanical Injury
  O’Grady KP, Kavanaugh TE, Cho H, Ye H, Gupta MK, Madonna MC, Lee J, O’Brien CM, Skala MC, Hasty KA, Duvall CL
  ACS Biomaterials Science & Engineering. 2017 Apr 6. DOI: 10.1021/acsbiomaterials.6b00804
• Dysfunctional BMPR2 signaling drives an abnormal endothelial requirement for glutamine in pulmonary arterial hypertension
  Egnatchik RA, Brittain EL, Shah AT, Fares WH, Ford HJ, Monahan K, Kang CJ, Kocurek EG, Zhu S, Luong T, Nguyen TT, Hysinger E, Austin ED, Skala MC, Young JD, Roberts LJ 2nd, Hemnes AR, West J, Fessel JP
  Pulm Circ. 2017 Feb 1;7(1):186-199. doi: 10.1086/690236.
• In vivo photothermal optical coherence tomography of endogenous and exogenous contrast agents in the eye
  Lapierre-Landry M, Gordon AY, Penn JS, and Skala MC
  Scientific Reports 7, Article number: 9228 (2017). doi:10.1038/s41598-017-10050-5
• Functional Optical Imaging of Primary Human Tumor Organoids for Personalized Drug Screens
  Walsh AJ, Cook RS, Skala MC
  J Nucl Med. (2017) Jun 6. doi: 10.2967/jnumed.117.192534.
• Autofluorescence imaging identifies tumor cell-cycle status on a single-cell level
  Heaster TM, Walsh AJ, Zhao Y, Hiebert SW, Skala MC
  J Biophotonics. (2017) May 9. doi: 10.1002/jbio.201600276
• Autofluorescence imaging captures heterogeneous drug response differences between 2D and 3D breast cancer cultures
  Cannon TM, Shah AT, Skala MC
  Biomed. Opt. Express (2017) 8, 1911-1925 doi: 10.1364/BOE.8.001911
• Metabolic Imaging of Head and Neck Cancer Organoids
  Shah AT, Heaster TM, Skala MC
  Metabolic Imaging of Head and Neck Cancer Organoids. (2017) PLoS ONE 12(1): e0170415. doi:10.1371/journal.pone.0170415

2016

• Copolymer-mediated cell aggregation promotes a proangiogenic stem cell phenotype in vitro and in vivo
  Crowder SW, Balikov DA, Boire TC, McCormack D, Lee JB, Gupta MK, Skala MC, Sung H-J
  Adv. Healthcare Mater. 2016 Sep.;  doi: 10.1002/adhm.201600819.
• Autofluorescence flow sorting of breast cancer cell metabolism
  Shah AT, Cannon TM, Higginbotham JN, Coffey RJ, Skala MC
  J. Biophotonics. 2016 Oct.;  doi: 10.1002/jbio.201600128.
• Depth-resolved analytical model and correction algorithm for photothermal optical coherence tomography
  Lapierre-Landry M, Tucker-Schwartz JM, Skala M
  Biomedical optics express. 2016; 7(7):2607-22.
• Optical Imaging of Drug-Induced Metabolism Changes in Murine and Human Pancreatic Cancer Organoids Reveals Heterogeneous Drug Response
  Walsh AJ, Castellanos JA, Nagathihalli NS, Merchant NB, Skala MC
  Pancreas. 2016 Jul;45(6):863-9. doi: 10.1097/MPA.0000000000000543.
• Temporal binning of time-correlated single photon counting data improves exponential decay fits and imaging speed
  Walsh AJ, Sharick JT, Skala MC, Beier HT.
  Biomedical optics express. 2016; 7(4):1385-99.
• Drug response in organoids generated from frozen primary tumor tissues
  Walsh AJ, Cook RS, Sanders ME, Arteaga CL, Skala MC
  Sci Rep. 2016 Jan 7;6:18889. doi: 10.1038/srep18889.

2015

• In Vivo Autofluorescence Imaging of Tumor Heterogeneity in Response to Treatment
  Shah AT, Diggins KE, Walsh AJ, Irish JM, Skala MC
  Neoplasia. 2015 Dec;17(12):862-70. doi: 10.1016/j.neo.2015.11.006.
• Deconvolution of fluorescence lifetime imaging microscopy by a library of exponentials
  Campos-Delgado DU, Navarro OG, Arce-Santana ER, Walsh AJ, Skala MC, Jo JA
  Opt Express. 2015 Sep 7;23(18):23748-67. doi: 10.1364/OE.23.023748.
• Signal Transducer and Activator of Transcription 3, Mediated Remodeling of the Tumor Microenvironment Results in Enhanced Tumor Drug Delivery in a Mouse Model of Pancreatic Cancer
  Nagathihalli NS, Castellanos JA, Shi C, Beesetty Y, Reyzer ML, Caprioli R, Chen X, Walsh AJ, Skala MC, Moses HL, Merchant NB
  Gastroenterology. 2015 Dec;149(7):1932-1943.e9. doi: 10.1053/j.gastro.2015.07.058. Epub 2015 Aug 7.
• Blind deconvolution estimation of fluorescence measurements through quadratic programming
  Campos-Delgado DU, Gutierrez-Navarro O, Arce-Santana ER, Skala MC, Walsh AJ, Jo JA
  J Biomed Opt. 2015 Jul;20(7):075010. doi: 10.1117/1.JBO.20.7.075010.
• Photothermal optical lock-in optical coherence tomography for in vivo imaging
  Tucker-Schwartz JM, Lapierre-Landry M, Patil CA, Skala MC
  Biomed Opt Express. 2015 May 27;6(6):2268-82. doi: 10.1364/BOE.6.002268. eCollection 2015 Jun 1.
• ROS-responsive microspheres for on demand antioxidant therapy in a model of diabetic peripheral arterial disease
  Poole KM, Nelson CE, Joshi RV, Martin JR, Gupta MK, Haws SC, Kavanaugh TE, Skala MC, Duvall CL
  Biomaterials. 2015 41:166-75. NIHMSID: NIHMS642415
• Optical metabolic imaging quantifies heterogeneous cell populations
  Walsh AJ, Skala MC
  Biomed Opt Express. 2015 Jan 15;6(2):559-73. doi: 10.1364/BOE.6.000559. eCollection 2015 Feb 1.
• Collagen density and alignment in responsive and resistant trastuzumab-treated breast cancer xenografts
  Walsh AJ, Cook RS, Lee JH, Arteaga CL, Skala MC
  J Biomed Opt. 2015 Feb;20(2):26004. doi: 10.1117/1.JBO.20.2.026004.
• High-throughput measurements of the optical redox ratio using a commercial microplate reader
  Cannon TM, Shah AT, Walsh AJ, Skala MC
  J Biomed Opt. 2015 Jan;20(1):010503. doi: 10.1117/1.JBO.20.1.010503.

2014

• Uncoupling angiogenesis and inflammation in peripheral artery disease with therapeutic peptide-loaded microgels
  Zachman AL, Wang X, Tucker-Schwartz JM, Fitzpatrick ST, Lee SH, Guelcher SA, Skala MC, Sung HJ
  Biomaterials. 2014; 35(36):9635-48. NIHMSID: NIHMS624426
• Quantifying the vascular response to ischemia with speckle variance optical coherence tomography
  Poole KM, McCormack DR, Patil CA, Duvall CL, Skala MC
  Biomedical optics express. 2014; 5(12):4118-30.
• Quantitative optical imaging of primary tumor organoid metabolism predicts drug response in breast cancer
  Walsh AJ, Cook RS, Sanders ME, Aurisicchio L, Ciliberto G, Arteaga CL, Skala MC
  Cancer research. 2014; 74(18):5184-94. NIHMSID: NIHMS617855
• In vivo hyperspectral imaging of microvessel response to trastuzumab treatment in breast cancer xenografts
  McCormack DR, Walsh AJ, Sit W, Arteaga CL, Chen J, Cook RS, Skala MC
  Biomedical optics express. 2014; 5(7):2247-61.
• In vivo imaging of nanoparticle delivery and tumor microvasculature with multimodal optical coherence tomography
  Tucker-Schwartz JM, Beavers KR, Sit WW, Shah AT, Duvall CL, Skala MC
  Biomedical optics express. 2014; 5(6):1731-43.
• The NADH oxidase ENOX1, a critical mediator of endothelial cell radiosensitization, is crucial for vascular development
  Venkateswaran A, Sekhar KR, Levic DS, Melville DB, Clark TA, Rybski WM, Walsh AJ, Skala MC, Crooks PA, Knapik EW, Freeman ML.
  Cancer research. 2014; 74(1):38-43. NIHMSID: NIHMS543105
• Optical metabolic imaging of treatment response in human head and neck squamous cell carcinoma
  Shah AT, Demory Beckler M, Walsh AJ, Jones WP, Pohlmann PR, Skala MC
  PloS one. PloS one. 2014; 9(3):e90746.

2013

• Quantitative optical imaging of vascular response in vivo in a model of peripheral arterial disease
  Poole KM, Tucker-Schwartz JM, Sit WW, Walsh AJ, Duvall CL, Skala MC
  American journal of physiology. Heart and circulatory physiology. 2013; 305(8):H1168-80.
• Optical metabolic imaging identifies glycolytic levels, subtypes, and early-treatment response in breast cancer
  Walsh AJ, Cook RS, Manning HC, Hicks DJ, Lafontant A, Arteaga CL, Skala MC
  Cancer research. 2013; 73(20):6164-74. NIHMSID: NIHMS515760
• The novel antiangiogenic VJ115 inhibits the NADH oxidase ENOX1 and cytoskeleton-remodeling proteins
  Venkateswaran A, Friedman DB, Walsh AJ, Skala MC, Sasi S, Rachakonda G, Crooks PA, Freeman ML, Sekhar KR
  Investigational new drugs. 2013; 31(3):535-44. NIHMSID: NIHMS413695
• Cross-sectional tracking of particle motion in evaporating drops: flow fields and interfacial accumulation
  Trantum JR, Eagleton ZE, Patil CA, Tucker-Schwartz JM, Baglia ML, Skala MC, Haselton FR
  Langmuir: the ACS journal of surfaces and colloids. 2013; 29(21):6221-31. NIHMSID: NIHMS792311
• Dual pH- and temperature-responsive microparticles for protein delivery to ischemic tissues
  Joshi RV, Nelson CE, Poole KM, Skala MC, Duvall CL
  Acta biomaterialia. 2013; 9(5):6526-34. NIHMSID: NIHMS445461
• Three-dimensional molecular imaging with photothermal optical coherence tomography
  Skala MC, Crow MJ, Wax A, Izatt JA.
  Methods in molecular biology. 2013; 1026:85-92. NIHMSID: NIHMS697574

2012

• In vivo photothermal optical coherence tomography of gold nanorod contrast agents
  Tucker-Schwartz JM, Meyer TA, Patil CA, Duvall CL, Skala MC
  Biomedical optics express. 2012; 3(11):2881-95.
• Ex vivo optical metabolic measurements from cultured tissue reflect in vivo tissue status
  Walsh AJ, Poole KM, Duvall CL, Skala MC
  Journal of biomedical optics. 2012; 17(11):116015.
• Dual-modality photothermal optical coherence tomography and magnetic-resonance imaging of carbon nanotubes
  Tucker-Schwartz JM, Hong T, Colvin DC, Xu Y, Skala MC
  Optics letters. 2012; 37(5):872-4. NIHMSID: NIHMS388212
• Optical imaging of metabolism in HER2 overexpressing breast cancer cells Walsh A, Cook RS, Rexer B, Arteaga CL, Skala MC
  Biomedical optics express. 2012; 3(1):75-85.

2004 – 2011

• Longitudinal optical imaging of tumor metabolism and hemodynamics
  Skala MC, Fontanella A, Lan L, Izatt JA, Dewhirst MW.
  Journal of biomedical optics. 2010; 15(1):011112.
• Multiphoton redox ratio imaging for metabolic monitoring in vivo
  Skala M, Ramanujam N
  Methods in molecular biology. 2010; 594:155-62. NIHMSID: NIHMS201646
• Combined hyperspectral and spectral domain optical coherence tomography microscope for noninvasive hemodynamic imaging
  Skala MC, Fontanella A, Hendargo H, Dewhirst MW, Izatt JA
  Optics letters. 2009; 34(3):289-91. NIHMSID: NIHMS122921
• Photothermal optical coherence tomography of epidermal growth factor receptor in live cells using immunotargeted gold nanospheres
  Skala MC, Crow MJ, Wax A, Izatt JA.
  Nano letters. 2008; 8(10):3461-7. NIHMSID: NIHMS68839
• In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia
  Skala MC, Riching KM, Gendron-Fitzpatrick A, Eickhoff J, Eliceiri KW, White JG, Ramanujam N
  Proceedings of the National Academy of Sciences of the United States of America. 2007; 104(49):19494-9.
• Comparison of a physical model and principal component analysis for the diagnosis of epithelial neoplasias in vivo using diffuse reflectance spectroscopy
  Skala MC, Palmer GM, Vrotsos KM, Gendron-Fitzpatrick A, Ramanujam N
  Optics express. 2007; 15(12):7863-75. NIHMSID: NIHMS24764
• In vivo multiphoton fluorescence lifetime imaging of protein-bound and free nicotinamide adenine dinucleotide in normal and precancerous epithelia
  Skala MC, Riching KM, Bird DK, Gendron-Fitzpatrick A, Eickhoff J, Eliceiri KW, Keely PJ, Ramanujam N
  Journal of biomedical optics. 2007; 12(2):024014. NIHMSID: NIHMS122928
• Multiphoton microscopy of endogenous fluorescence differentiates normal, precancerous, and cancerous squamous epithelial tissues
  Skala MC, Squirrell JM, Vrotsos KM, Eickhoff JC, Gendron-Fitzpatrick A, Eliceiri KW, Ramanujam N
  Cancer research. 2005; 65(4):1180-6. NIHMSID: NIHMS485459
• Investigation of fiber-optic probe designs for optical spectroscopic diagnosis of epithelial pre-cancers
  Skala MC, Palmer GM, Zhu C, Liu Q, Vrotsos KM, Marshek-Stone CL, Gendron-Fitzpatrick A, Ramanujam N.
  Lasers in surgery and medicine. 2004; 34(1):25-38. NIHMSID: NIHMS122929