Rhoads, Tim |
Assistant Professor | Email | Website | Nutritional Sciences | (503) 475-1970 | My lab is broadly interested in the molecular regulation of age-related changes to metabolism. We investigate mechanisms of gene expression regulation at the transcriptional, translational, and post-translational levels, to identify and understand drivers of metabolic change that may underlie the development of age-associated chronic diseases. |
Meyer, Mark |
Assistant Professor | Email | Website | Nutritional Sciences | (608) 890-0857 | The Meyer lab studies the dynamic chromatin environment responsible for serum calcium and phosphate maintenance and the impacts of vitamin D metabolism in skeletal, renal, and intestinal biology. A triumvirate of endocrine hormones – parathyroid hormone (PTH), fibroblast growth factor 23 (FGF23), and calcitriol (1,25(OH)2D3) – maintain this delicate balance by influencing enzymes, transporters, and transcription factors to drive genomic change. When dysfunctional, these mechanisms allow chronic inflammation and disease progression to worsen in chronic kidney disease-metabolic bone disorder (CKD-MBD), atherosclerosis, inflammatory bowel disease (IBD), and many others. Dietary and nutritional supplementation of vitamin D rapidly corrects the body’s mineral deficiencies, however its ability to ameliorate inflammatory disease progression remains controversial. We study the intricate genomic and molecular mechanisms that regulate the biological changes controlling the intersection of metabolism, inflammation, and disease progression using unique animal models, genomic editing techniques, and -omics bioinformatic approaches to generate unbiased interrogation of chromatin changes. |
Overmyer, Katherine |
Associate Director, LBMS | Email | Website | Biomolecular Chemistry; Morgridge Institute for Research | (608) 262-4211 | I collaborate with researchers across campus to facilitate mass spectrometry based omics analysis through the Laboratory for Biomolecular Mass Spectrometry (LBMS). My scientific interests are on improving methodologies related to multi-omics data acquisition and integration. |
Zhao, Xinyu |
Professor | Email | Website | Neuroscience | (608) 263-9906 | My lab is investigating the impact of metabolic and mitochondrial deficits on stem cell differentiation, brain development, and neurodevelopmental disorders, including autism, fragile X syndrome, and schizophrenia. |
Merrins, Matthew |
Associate Professor | Email | Website | Medicine; Biomolecular Chemistry | (608) 256-1901 | My lab is focused on understanding oscillatory metabolic signaling in the pancreatic islet and its deficiency in type 2 diabetes. My lab specializes in single-cell approaches – principally live-cell imaging and electrophysiology – and since the lab’s inception we have developed a number of exciting tools which have given us new insights into diabetes pathophysiology. One example is fluorescence lifetime imaging of NADH (FLIM-N), which allows us to distinguish metabolic activity in each subcellular compartment of the pancreatic beta cell, including the mitochondria. Using FLIM-N and FRET imaging, we hope to understand the bidirectional communication between mitochondrial metabolism and the cell cycle machinery, mediated by cyclin dependent kinases. We are also focused on characterizing a number of mitochondrial proteins initially identified by RNA-seq as type 2 diabetes-associated loci; in this case, we are using Phy-PIF optogenetics to elucidate the mechanisms by which these proteins alter mitochondrial dynamics, metabolic oscillations, and insulin secretion. |
Eisenstein, Rick |
Professor | Email | Website | Nutritional Sciences | (608) 262-5830 | We study mechanisms of iron sensing and control of iron homeostasis in vertebrates by iron regulated RNA binding proteins, the iron regulatory proteins (IRP). IRP maintain iron homeostasis by controlling the fate of mRNA encoding proteins needed for iron metabolism or the responses to iron deficiency. We investigate how iron metabolism and erythropoiesis is coordinated particularly how IRP1 senses iron or oxygen status and controls the translation of hypoxia inducible factor 2-α (HIF-2α) mRNA. HIF-2α, a transcription factor, promotes adaptive responses to hypoxia by enhancing both red blood cell production and dietary iron acquisition for hemoglobin production. We use animal, cell culture and model systems (yeast) to define the physiological roles of IRP1, its selective control of mRNA fate and the iron trafficking pathways it responds to. Genome editing, flow cytometric analysis of hematopoiesis, RNA binding, gene by diet interaction studies, tissue specific knockouts and in vivo imaging. |
Keller, Mark |
Distinguished Scientist | Email | Website | Biochemistry | (608) 263-4234 | I have worked closely with Professor Alan Attie for more than 16 years to integrate genetics, physiology and metabolism to better understand the molecular basis of disease susceptibility. The lab exploits natural genetic variation contained within in-bred mouse strains as a platform to link metabolic disease with genetics. One major project is focused on surveying diet-induced metabolic syndrome in a newly developed mouse resource called the Diversity Outcross (DO). Each DO mouse derives from 8 distinct parental mouse strains that together represent the genetic diversity contained within the human population. The degree of metabolic syndrome we observe in the DO population is remarkable, illustrating that phenotypic variation is driven by genetic variation. We employ various omics-based measurements (e.g., transcriptomics, proteomics, microbiomics, metabolomics) to generate robust causal models that will help to unravel the enormously complex relationship between the genetics of each DO mouse and their relative susceptibility to metabolic syndrome. |
Yen, Eric |
Associate Professor | Email | Website | Nutritional Sciences | (608) 890-1888 | We are interested in understanding how cellular metabolism modulates systemic energy balance in response to diet. Our research centers on the synthesis of triacylglycerol, which serves as a storage and transport molecule of bioactive fatty acids and excess calories. Using genetically engineered mice, we examine the physiological functions of enzymes involved in the process. One current focus is on monoacylglycerol acyltransferase 2, which mediates the absorption of dietary fat in the small intestine. Mice lacking the enzyme are protected against obesity and other metabolic disorders normally induced by high-fat feeding. Interestingly, these mice absorb a normal quantity of fat but exhibit increases in energy expenditure. We are now combining biochemical and systems biology approaches to understand the underlying molecular mechanisms. The ultimate goals are to better understand the fundamental process of fat assimilation and to explore new approaches to prevent obesity and other metabolic diseases associated with excessive energy storage. |
Engin, Feyza |
Associate Professor | Email | Website | Biomolecular Chemistry; Medicine | (608) 262-8667 | Our lab is interested in understanding the role of organelle stress and stress responses in disease pathogenesis. The endoplasmic reticulum (ER) orchestrates protein synthesis, folding and trafficking in the cell and disruption of the ER's adaptive capacity results in activation of the unfolded protein response (UPR). Under chronic stress conditions, UPR engage many different inflammatory and stress signaling pathways that are critical for disease pathologies including insulin resistance, obesity and type2 diabetes. Interestingly, we recently discovered that ER stress plays a significant role in the pathogenesis of not only type 2 diabetes, but also autoimmune diabetes. Currently, we are exploring the molecular mechanisms leading to ER stress in type 1 diabetes and investigating the role of different UPR branches in metabolic homeostasis. We are also investigating how disruption of ER calcium homeostasis may affect interactions between ER and mitochondria in the context of metabolic and autoimmune diseases. |
Hernandez, Laura |
Professor | Email | Website | Animal and Dairy Sciences | (608) 263-9867 | Our research is focused on how serotonin controls maternal metabolism to support lactation. We focus on two areas of how the mammary gland controls maternal metabolism during lactation: calcium homeostasis and energy homeostasis. We utilize rodent and cow in vivo models and as well in vitro techniques. |
Knoll, Laura |
Professor | Email | Website | Medical Microbiology and Immunology | (608) 262-3161 | Our research centers on studying the host/pathogen interactions for the intracellular parasite Toxoplasma gondii. Toxoplasma is a highly successful parasite that exists as a life-long infection in a large percentage of the world’s warm-blooded animals, including almost half the human population. In a healthy individual, Toxoplasma has evolved to stimulate, but not over-stimulate the host’s immune response. Toxoplasma causes encephalitis in immunocompromised patients and is a member of the coccidian family that includes Plasmodium. We are combining next generation sequencing, proteomics and metabolomics to uncover the host and parasite metabolic pathways that are necessary for Toxoplasma to establish and maintain chronic infection. Because Toxoplasma is auxotrophic for many essential nutrients, we are finding that infection dramatically manipulates host cell metabolism. Toxoplasma also has both a mitochondria and a remnant chloroplast, so it has several unusual metabolic products. These studies will generate new anti-parasitic targets that will help develop novel therapies. |
Coe, Christopher |
Emeritus Professor | Email | Website | Psychology | (608) 263-3550 | My research spans a number of topics in behavioral medicine and health psychology, which involve metabolism. Using a nonhuman primate model, we are investigating the relationship between the gut microbiome and systemic physiology during infancy. We also are studying novel iron supplements to treat iron deficiency anemia, and include metabolomic approaches to assess the benefits of treatment. Our research with human participants is focused more on the other end of the life span, and the biology of aging. My lab oversees the biomarker assessments for 2 surveys of health and aging in the US and Japan (MIDUS and MIJDA). Both include a number of outcomes related to metabolism, especially with respect to obesity and glucoregulation. |
Sutula, Thomas |
Emeritus Professor | Email | Website | Neurology | (608) 263-5448 | My interest and ongoing work addresses the influences of glycolysis and metabolism on neuronal and circuit function in the brain. |
Converse, Alexander |
Senior Scientist | Email | Website | Waisman Center | (608) 265-6604 | Positron emission tomography (PET) using [18F]fluorodeoxyglucose (FDG) to noninvasively image glucose metabolism |
Baker, Mei |
Co-Director, Newborn Screening Laboratory | Email | Website | Wisconsin State Laboratory of Hygiene | (608) 890-1796 | My research interest is public health genetics and genomics, with a focus on applying and translating advanced biochemical and molecular technologies into routine newborn screening practice to enable public health laboratories to screen for new conditions and improve screening performance for the exiting screened conditions. |
Donohue, Timothy |
Director, WEI; Professor | Email | Website | Bacteriology | (608) 262-4663 | Bacterial metabolism and energy generation, this include central carbon and nitrogen metabolism, aromatic utilization and control of these pathways. We use genetic, biochemical genomic and other approaches in a wide set of microbes, but pure cultures and consortia or simple microbiomes |
Fan, Jing |
Assistant Professor; Investigator | Email | Website | Nutritional Sciences; Morgridge Institute for Research | Our lab is interested in understanding how mammalian cellular metabolism is reprogrammed in response to changes in the environment and cellular state, and how activities in key metabolic pathways can in turn affect cell function. To study this, we combine systems biology approaches, especially fluxomics and metabolomics, with computational modeling and biochemical and genetic techniques. Particularly, our current works focus on (1) understanding the metabolic adaptations in cancer cells in acidic microenvironment, and (2) investigating the metabolic regulation during macrophage polarization |
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Eide, David |
Professor | Email | Website | Nutritional Sciences | (608) 263-1613 | My lab studies the mechanisms cells use to respond and adapt to metal nutrient deficiencies. We study these processes in the yeast Saccharomyces cerevisiae and focus primarily on zinc deficiency. In this yeast, the Zap1 transcription factor responds to zinc deficiency to activate the expression of ~80 genes. The products of these genes include several zinc transporters responsible for zinc homeostasis. Many Zap1-regulated genes also encode proteins involved in the adaptation to zinc deficiency including proteins involved in oxidative stress resistance, protein homeostasis, sulfur metabolism, and phospholipid synthesis. |
Newton, Michael |
Professor; Department Chair | Email | Website | Biostatistics and Medical Informatics | (608) 263-0357 | I do statistics and biostatistics, especially in genomic apps, and have collaborated on various projects that integrate different data sources |
Nelson, David |
Emeritus Professor | Email | Website | Biochemistry | (608) 263-6879 | I am interested in elucidating the interaction between cellular processes and whole body energy metabolism. In our research group, we use molecular genetics to modulate the function of genes involved in lipid metabolism. My primary focus is on the role of intestinal monoacylglycerol acyltransferase 2 (MGAT2), an enzyme that catalyzes the conversion of MAG to DAG (an essential step in the efficient absorption and assimilation of dietary fat), in mediating systemic responses to diet. Using indirect calorimetry, I am linking alterations in food intake, oxygen consumption (VO2), and substrate utilization (RER) with diet and environmental challenges in genetically engineered mice. In particular, I am investigating MGAT2’s role in intracellular lipid trafficking and effects on enteroendocrine signaling and the kinetics of nutrient absorption and delivery. Through this research, we hope to better understand how alterations in the kinetics of intestinal lipid metabolism affect whole body energy metabolism, obesity, and related morbidities. |
Jefcoate, Colin |
Emeritus Professor | Email | Website | Cell and Regenerative Biology | (608) 263-3975 | The Laboratory addresses signaling processes involved in adaptation to Diet, Chemicals and Stress. Mesenchymal cells are key mediators, which include multi-potential progenitor cells that deliver physical structure, energy control (adipose), steroid production and local support factors. The Laboratory played key roles in the discovery of two regulators of these processes; Cytochrome P450 1B1 (CYP1B1) and the Steroidogenesis Acute Regulator (StAR). CYP1B1 is expressed in mesenchymal progenitors and vascular cells, but also controls oxidative stress, vascular adhesion and monocyte differentiation. We probe CYP1B1 functions through general and selective deletions of CYP1B1 from mice, facilitated by the development of a flox/flox Cyp1b1 mouse (gene expression/associated physiological changes). Hormonal activation of StAR, a labile protein, directs delivery of cholesterol to Cyp11a1 in mitochondria, thereby initiating adrenal glucocorticoid synthesis, a mediator of liver energy homeostasis or testosterone synthesis in fetal and adult testis, a notable source of endocrine disruption. |
Fox, Brian |
Professor; Department Chair | Email | Website | Biochemistry | (608) 262-9708 | Protein structure and function. Oxidation and reduction reactions. Fatty acid metabolism. Production of biofuels and other products from cellulosic biomass. |
Colopy, Sara |
Clinical Assistant Professor | Email | Website | Surgical Sciences | (608) 262-4580 | My laboratory is focused on understanding the pathogenesis of urinary tract infection and bladder epithelial healing. One current area of research is looking at the pathogenesis of urinary tract infections in type II diabetic mice. |
Landick, Robert |
Professor | Email | Website | Biochemistry; Bacteriology | (608) 265-8475 | We study the regulation of RNA synthesis by RNA polymerase and the use of gene expression engineering to create novel microbial systems and communities to produce beneficial compounds and materials. These studies link to metabolic regulation through a diverse set of protein, RNA, and small molecule regulators that interact with RNA polymerase to control gene expression, in the search for new inhibitors of RNA polymerase as lead compounds for drug discovery, and via ways that changes in gene expression can redesign metabolism for useful purposes. |
Denu, John |
Professor, Epigenetics Theme Leader | Email | Website | Biomolecular Chemistry; Wisconsin Institute for Discovery | (608) 316-4341 | Linking metabolism with the epigenome: Chromatin remodeling enzymes rely on co-enzymes derived from metabolic pathways, suggesting coordination between nuclear events and metabolic networks. Investigations are underway to understand the link between metabolism and the regulation of epigenetic mechanisms. We are testing the hypothesis that certain chromatin modifying complexes have evolved to exquisitely ‘sense’ metabolite levels and respond accordingly, modifying specific chromatin loci for altered gene expression. Sirtuins and reversible protein acetylation: Accumulating evidence suggests that reversible protein-lysine is a major regulatory mechanism that controls non-histone protein function. Sirtuins are a conserved family of NAD+-dependent protein deacetylases that have emerged as important players in modulating protein acetylation. Compelling genetic evidence implicates sirtuins in genome maintenance, metabolism, cell survival, and lifespan. The NAD+-dependence suggests that specific protein deacetylation is inextricably linked to metabolism. We are examining the central hypothesis that reversible protein acetylation is a major regulatory mechanism for controlling diverse metabolic processes, and that at the molecular level, site-specific acetylation alters the intrinsic activity of targeted proteins. |
Markley, John |
Emeritus Professor | Email | Website | Biochemistry | (608) 262-3173 | Our major interest is in NMR-based investigations of metabolites and their interactions. NMR allows unbiased detection and quantification of the 30-80 most abundant metabolites in biological fluids and tissue extracts. We created a database of one- and two-dimensional spectra of metabolites and other small molecules of biological importance, which has grown to include ~1400 compounds. We developed efficient technology for high-throughput data collection, automated assignment and quantification of individual species and visual inspection of the results. We recently developed a platform for rapid screening of proteins against a panel of 500 metabolites to determine whether the protein binds or chemically modifies these small molecules. In favorable cases, we can use NMR to validate hits and to determine ligand binding sites. In the realm of natural products, we provide technology for LC-MS with solid phase extraction so that molecules with a mass of interest can be isolated for subsequent NMR analysis. |
Lamming, Dudley |
Associate Professor | Email | Website | Medicine | (608) 262-7341 | The Lamming laboratory's goal is to understand how nutrient-responsive signaling pathways can be harnessed to promote health and longevity. We are primarily focused on the physiological role played by the mechanistic target of rapamycin (mTOR), a protein kinase that through a diverse set of substrates regulates complex cellular processes, including growth, metabolism, and aging. Recent work has shown that rapamycin, an inhibitor of mTOR signaling, can improve both health and longevity in model organisms including mammals. Understanding and manipulating the mTOR signaling pathway through dietary, pharmaceutical or genetic interventions in mouse models may provide insight into the treatment of age-related diseases, including diabetes, Alzheimer's disease, cancer, and Hutchinson-Gilford Progeria Syndrome. Learn more at http://www.lamminglab.org/ |
Alexander, Caroline |
Professor | Email | Website | Oncology | (608) 265-5182 | We aim to determine the role of a skin-associated lipid layer, we call dermal white adipose tissue, on mammalian insulation, and therefore on glucose disposition and the frequency of thermogenic activation of brown adipose tissues. We propose that manipulation of this dWAT layer could confer health benefits to human subjects. Our research is enabled by the development of a high resolution, body wide quantitative MRI technique |
Dowell, James |
Associate Scientist | Email | Website | Biomolecular Chemistry; Wisconsin Institute for Discovery | (608) 306-4449 | My research is focused on the metabolism of the brain. Specifically, I am interested in metabolic processing of glutamate in astrocytes, its regulation by protein kinases, and its involvement in neurodegeneration. I use both mass spectrometry-based metabolomics and proteomics platforms to study these processes. |
Westmark, Cara |
Assistant Professor | Email | Website | Neurology | (608) 262-9730 | My research interests lie in the area of synaptic function as related to the over-expression of amyloid-beta protein precursor and amyloid-beta in Alzheimer’s disease, Down syndrome and fragile X syndrome. I study therapeutic approaches that reduce amyloid-beta and rescue seizure, behavioral, cognitive and biomarker phenotypes associated with the aforementioned disorders. During the course of these studies, I serendipitously discovered that diet, specifically soy-based diets, exacerbate seizure incidence and weight gain in juvenile mice as well as in infants. Soy is rich in phytoestrogens and contaminated with agrochemicals, which can act as endocrine disrupting chemicals. Surprisingly, there has been a paucity of studies regarding the long-term effects of consuming singe-source soy-based diets. Our research focus in metabolism is to study the effect of soy on neurological and metabolic phenotypes, particularly in developmental disability models, to validate dietary restriction of soy-based infant formula as a therapeutic intervention for autism and fragile X. |
Li, Lingjun |
Professor | Email | Website | Pharmaceutical Sciences Division | (608) 265-8491 | Our research focuses on the development of mass spectrometry-based tools and systems biology strategy to understand metabolic profile changes during various disease conditions such as aging, lower urinary tract symptoms, and cardiovascular injury. We also employ imaging MS technology to understand symbiosis between bacteria/microbiome and host organisms. |
Dahlberg, James |
Emeritus Professor | Email | Website | Biomolecular Chemistry | (608) 262-1459 | Impact of physiology on metabolic states. |
Suen, Garret |
Associate Professor | Email | Website | Bacteriology | (608) 890-3971 | Our work focuses on understanding the rumen ecosystem with an toward improving animal production and utilizing this microbiome as a model for biofuel production. We utilize genome-enabled approaches to characterize and understand this community to determine if alterations to the rumen microbiome can result in increased animal production. From a biofuels perspective, our work seeks to determine how this highly optimized microbiome is capable of rapid biomass degradation and fermentation. |
Keller, Nancy |
Professor | Email | Website | Bacteriology; Medical Microbiology and Immunology | (608) 262-9795 | My lab focuses on the genetics and chemistry of fungal natural products (NPs). We explore the role of NPs as virulence factors (particularly in Aspergillus and Penicillium species), as signaling molecules in intra- and inter-Kingdom milieus and for development as pharmaceuticals. We are interested in the crosstalk of primary and secondary metabolism in fungi and how natural product clusters are regulated by endogenous mechanisms (e.g. epigenetic regulation and global transcriptional regulators) or exogenous input from other organisms (e.g. other microbes or hosts) and abiotic factors. |
Sinha, Divya |
Scientist I | Email | Website | Waisman Center | (608) 263-1656 | Human pluripotent stem cell derived retinal pigment epithelium (hPSC-RPE) and photoreceptor (hPSC-PR) cells are being utilized for in vitro modeling of retinal diseases as well as to develop cell replacement therapy for retinal degenerative diseases that lead to blindness. Since these retinal cell types are highly functional and mitochondria-rich in vivo, our efforts are focused on assessing and enhancing cell health of hPSC-derived RPE and photoreceptors in terms of mitochondrial metabolism under physiologic conditions. |
Eells, Janis |
Professor | Email | Website | Biomedical Sciences at UW-Milwaukee | (414) 229-2645 | Mitochondria play a key role in cellular metabolism and intracellular signaling. Mitochondrial dysfunction and the resulting oxidative stress are central in the pathogenesis of aging and degenerative diseases including diabetes, cardiovascular disease, macular degeneration and Alzheimer’s disease. Research in my laboratory is directed at understanding the mitochondrial signaling pathways that regulate the processes of cellular aging and degeneration with the long-term goal of learning how to protect cells and tissues against these degenerative processes. One mitoprotective strategy is photobiomodulation. Exposure to low energy photon irradiation in the far-red (FR) to near-infrared (NIR) range of the spectrum (630 – 900 nm), collectively termed “photobiomodulation” (PBM) can restore the function of damaged mitochondria, upregulate the production of cytoprotective factors and prevent apoptotic cell death. FR/NIR photons penetrate diseased tissues including the retina and optic nerve. Investigations in rodent models of retinal injury and disease have demonstrated the PBM attenuates photoreceptor cell death, protects retinal function and exerts anti-inflammatory actions. Recent clinical studies have documented amelioration of atrophic AMD. |
Maeda, Hiroshi |
Professor | Email | Website | Botany | (608) 262-5833 | The Maeda lab investigates how plants synthesize aromatic amino acids, which are essential nutrients in the human diet and key precursors of numerous plant natural products (e.g. alkaloids, quinones, phenolic compounds). We combine phylogenetic, biochemical, genetics, and protein structure analyses to understand how key enzymes in the aromatic amino acid pathways evolved in different plant lineages that produce distinct downstream specialized metabolites. Such evolutionary variations are then utilized to identify amino acid residues responsible for their catalytic and regulatory properties and also to enhance the production of natural products through metabolic engineering. |
Gamm, David |
Professor | Email | Website | Ophthalmology and Visual Sciences | (608) 261-1516 | Human pluripotent stem cell derived retinal pigment epithelium (hPSC-RPE) and photoreceptor (hPSC-PR) cells are being utilized for in vitro modeling of retinal diseases as well as to develop cell replacement therapy for retinal degenerative diseases that lead to blindness. Since these retinal cell types are highly functional and mitochondria-rich in vivo, our efforts are focused on assessing and enhancing cell health of hPSC-derived RPE and photoreceptors in terms of mitochondrial metabolism under physiologic conditions. |
Cryns, Vincent |
Professor | Email | Website | Medicine | (608) 262-4786 | Tumor cells must adapt to metabolic stress intrinsic to their rapid growth in order to survive. Our group focuses on the molecular mechanisms by which tumor cells adapt to metabolic stress. We are also interested in targeting metabolic stress in tumor cells by depriving them of essential nutrients to metabolically prime them to respond to pro-apoptotic therapies. As a defining feature of cancer cells, metabolic stress offers unprecedented translational opportunities to selectively target cancer. |
Parks, Brian |
Assistant Professor | Email | Website | Nutritional Sciences | (608) 262-3445 | The Parks Lab is focused on addressing the question of how genetics and diet interact together to contribute to common metabolic diseases, such as obesity and diabetes. Through the use of large-scale integrative genetic studies in the mouse we have identified several candidate genes that mediate gene-diet interactions. Current work is focused on a novel candidate drug target, Agpat5, which improves common symptoms of obesity and diabetes. In addition to this work we have a strong interest in the development of systems genetics approaches for dissecting biological pathways and networks. |
Brow, Dave |
Professor | Email | Website | Biomolecular Chemistry | (608) 262-1475 | Metabolite-regulated gene expression in yeast. We identified a pathway for regulation of IMPDH synthesis by its end-product GTP via transcription attenuation. We are investigating additional levels of regulation using IMPDH-GFP and quantitative live-cell microscopy. Mutations in human IMPDH1 can result in an inherited form of blindness, retinitis pigmentosa, possibly by disrupting binding of IMPDH to its gene or mRNA. We are using yeast as a model system to study the effects of these disease mutations. |
Amador-Noguez, Daniel |
Associate Professor | Email | Website | Bacteriology | (608) 265-2710 | The production of biofuels from cellulosic biomass holds promise as a source of clean renewable energy that can reduce our dependence on fossil fuels. Attaining this goal will require engineered microorganisms capable of economical conversion of cellulosic biomass into biofuels. Effective microbe design relies on understanding the relevant metabolic pathways and their regulation, including how the integrated networks function as a whole. My research program integrates systems-level analyses, especially metabolomics, with computational modeling and genetic engineering to advance understanding of metabolism in biofuel producing microorganisms, particularly clostridium species such as C. acetobutylicum, C. cellulolyticum and C. thermocellum. The main research topics in my laboratory are: 1) Systems-level analysis of metabolic regulation in biofuel producing microorganisms and 2) Engineering symbiotic consortia for biofuel production. |
Burkard, Mark |
Professor | Email | Website | Medicine | (608) 262-2803 | My research is focused on identifying unique abnormalities of cancer cells, including metabolic alterations, that confer sensitivity to anticancer therapy, thereby enabling precision medicine. |
Hittinger, Chris |
Professor | Email | Website | Genetics | (608) 265-6664 | We study the evolutionary genomics of yeasts with particular emphases on biodiversity and carbon metabolism. Over the last half a billion years, different yeast species have evolved radically different metabolic strategies, from the rare highly fermentative lifestyle of Saccharomyces (i.e. Crabtree-Warburg Effect or aerobic fermentation) to yeasts that accumulate over half of their dry weight as fatty acids. We use genetic, genomic, phylogenetic, and metabolic approaches to understand how these differences are encoded in their genomes. We also have applied projects in brewing, cellulosic biofuels, and synthetic biology. |
Cobra, Paulo Falco |
Instrument Director | Email | Website | NMRFAM; Biochemistry | (608) 265-3303 | The main project I'm currently working on focus on the investigation of two different species of the protozoa responsible for the leishmaniasis disease and how their metabolism is affect by adding two of the current drugs used to treat the disease to the growth medium used to cultivate them. There are other collaboration projects in different segments being investigated simultaneously. |
Kemnitz, Joseph |
Professor | Email | Website | Cell and Regenerative Biology | (608) 263-9726 | calorie restriction and aging; T2DM |
Audhya, Anjon |
Professor; Senior Associate Dean | Email | Website | Biomolecular Chemistry | (608) 262-3761 | The Audhya lab is interested in the contribution of membrane transport pathways (both secretory and endocytic trafficking) to cellular metabolism as it relates to growth, development, endocrine function, and the regulation of lipid homeostasis. |
Kimple, Michelle |
Associate Professor | Email | Website | Medicine | (608) 265-5627 | G protein-coupled receptor signaling pathways affecting pancreatic beta-cell function, growth, and survival in normal and pathophysiological states. |
Bates, Donna |
Research Support Coordinator | Email | Website | Great Lakes Bioenergy Research Center | (608) 890-4843 | I work in the Conversion Area of GLBRC where I help coordinate research on making biofuels from lignocellulosic biomass. This research relies heavily on an understanding of microbial metabolic pathways and ways in which they can be manipulated. |
Puglielli, Luigi |
Professor | Email | Website | Medicine | (608) 256-1901 | Role of the endoplasmic reticulum (ER) acetylation machinery and intracellular acetyl-CoA flux in developmental and degenerative diseases. |
Craven, Mark |
Professor | Email | Website | Biostatistics and Medical Informatics | (608) 265-6181 | Developing and applying methods from machine learning, natural language processing, and optimization to infer models characterizing networks of interactions among genes, proteins, metabolites, clinical variables, environmental factors, and phenotypes of interest. |
Livny, Miron |
Lead Investigator, Research Computing | Email | Website | Morgridge Institute for Research | (608) 316-4336 | Distributed High Throughput Computing. Frameworks and software tools that enable researchers to run with the help of automation tools (workflows) large ensembles of interdependent jobs on large collection of distributed computing and data resources. |
Sato, Trey |
Senior Scientist | Email | Website | Great Lakes Bioenergy Research Center | (608) 890-2546 | My group is interested in understanding the regulation of lignocellulosic sugar conversion into biofuels and bioproducts by Saccharomyces cerevisiae. We employ metabolomic, proteomic and transcriptomic tools to determine the molecular mechanisms by which genetically engineered and evolved yeast strains increase their rate of sugar metabolism. |
Reeder, Scott |
Professor | Email | Website | Radiology | (608) 262-0135 | My research interests focus on the development and validation of advanced magnetic resonance imaging (MRI) methods to quantify tissue characteristics such as tissue triglyceride and iron concentration. Our group also works to develop methods to quantify body composition, including visceral and subcutaneous adipose tissue volumes, total body fat and muscle volumes. Many of these methods can be performed in children and adults, as well as animal models such as mice, rats, and large animals. |
Aceti, David |
Scientist | Email | Website | Biochemistry | (608) 890-0171 | Ligand screening to assign functions to orphan proteins |
Ponik, Suzanne |
Assistant Professor | Email | Website | Cell and Regenerative Biology | (608) 265-2398 | Increased collagen density and organization are driving factors for breast cancer progression. However, the specific cellular mechanisms resulting in altered cell metabolism, proliferation and invasion are not yet clearly defined. I am interested in identifying the mechanisms involved in altered cellular metabolism in a dense collagen microenvironment. |
Stewart, Ron |
Investigator, Bioinformatics | Email | Website | Morgridge Institute for Research | (608) 316-4349 | We are interested in the relationship between metabolism and stem cell differentiation. |
Karasov, William |
Emeritus Professor | Email | Website | Forest and Wildlife Ecology | (608) 263-9319 | We study metabolism of vertebrate wildlife. The majority of the studies involve measurement of whole-animal metabolism (indirect calorimetry), often using the doubly labeled water method. These measurements provide insights into nutrition, feeding and population ecology of wildlife and also their exposure to toxicants in foods. We also study digestive physiology, including work related to gut microbiome. |
Roy, Sushmita |
Associate Professor | Email | Website | Biostatistics and Medical Informatics | (608) 316-4453 | My research generally spans the development and application of computational methods based on machine learning for inference and analysis of different types of molecular regulatory networks. Specifically we are interested in developing methods that enable us to ask three main questions: (A) what networks exist in a specific biological context (e.g. a cell type, tissue, species), (B) how do they change between cell types and how do they evolve across species, and (C) how do changes in the network affect overall cellular and organismal state. |
Bugni, Tim |
Professor | Email | Website | Pharmaceutical Sciences Division | (608) 263-2519 | Bacteria produce a large repertoire of small molecules (<2,000 Da) that modulate a wide variety of biological targets. Many of these molecules have found therapeutic applications, especially in the areas of cancer and infectious disease. While genomic studies on bacteria have clearly shown that many more molecules remain undiscovered, finding new molecules has become akin to finding the needle in the haystack. We develop and apply LCMS-based metabolomics methods to greatly improve discovery rates by finding the needle in the haystack. We also use a combination of genomics and metabolomics to understand how bacterial interactions lead to production of new antibiotics. These strategies are yielding promising results in terms of generating new antifungal and antibiotic agents. |
Arriola Apelo, Sebastian |
Assistant Professor | Email | Website | Animal and Dairy Sciences | (608) 262-5129 | The mechanistic Target of Rapamycin Complex 1 (mTORC1) is a central hub of nutritional and hormonal signals that regulates a wide range of metabolic processes, proteostasis among them. We study the role of mTORC1, on the regulation of protein synthesis, degradation and secretion in mammary epithelial cells, as mean of understanding milk protein production. We use nutritional, pharmacological and genetic approaches to quantitatively determine the response to different metabolic signals. The final objective is to determine accurate nutrient requirements for milk protein production and minimize the environmental impact of the dairy industry. |
Attie, Alan |
Professor | Email | Website | Biochemistry | (608) 262-1372 | My lab studies pathways leading to metabolic diseases, especially diabetes and hyperlipidemia. We use genetics to discover novel genes and pathways leading to metabolic disease. For us, genetics provides a powerful means to establish causal connections and discover complex pathways. Much of our work on diabetes is focused on beta-cells, the cells in the endocrine pancreas that sense nutrients and secrete insulin. Our work has identified novel proteins involved in nutrient sensing, intracellular signaling, and vesicle trafficking. |
Chang, Qiang |
Director, Waisman Center; Professor | Email | Website | Medical Genetics and Neurology | (608) 262-9416 | My lab uses mouse models and human stem cell models to study neurodevelopmental disorders, including Rett syndrome (RTT). Recently, we have identified several mitochondrial defects in astrocytes differentiated from RTT patient-specific induced pluripotent stem cells (iPSCs). We are currently investigating the molecular and cellular events linking RTT mutations and mitochondrial dysfunction, and screening for drugs that reverse the mitochondrial defects in RTT. |
Chung, Moo K. |
Associate Professor | Email | Website | Biostatistics and Medical Informatics | (608) 217-2452 | Methodological issues (computational, statistical, mathematical) on modeling PET scans of human brain at both the voxel-level and the network-level (metabolic connectivity). |
Clagett-Dame, Margaret |
Emeritus Professor | Email | Website | Biochemistry | (608) 262-3450 | Our metabolism work is focused on the absorption, distribution, metabolism and elimination of 2-methylene-19-nor vitamin D analogs that are active in skin. We administer radiolabeled compound to mice and evaluate total radioactivity over time in a variety of tissues, as well as the presence of intact compound and metabolites after extraction and separation by HPLC. Our goal is to use this information to better understand the biological activity and toxicity profile for these compounds. |
Coon, Josh |
Professor | Email | Website | Chemistry and Biomolecular Chemistry | (608) 263-1718 | Our laboratory develops and applies mass spectrometric technology to study human health. We use these tools to answer fundamental questions in cell biology and to study human diseases including Alzheimer’s, diabetes, heart failure, cancer, obesity, asthma, among several others. The research team currently consists of twelve Ph.D. students, two postdoctoral fellows, two undergraduate researchers, and five staff scientists. |
Craig, Betty |
Professor | Email | Website | Biochemistry | (608) 263-7105 | Our lab studies the roles of molecular chaperones in complex biological processes, ranging from general protein folding to propagation of yeast prions. We have a particular interest in mitochondrial physiology and metabolism, because - an Hsp70 molecular chaperone forms the core of the import motor required for translocation of proteins from the cytosol into the mitochondrial matrix; an Hsp70 is required for the biogenesis of Fe-S clusters, essential co-factors of a number of key metabolic enzymes and components of the respiratory chain. |
Davis, Dawn Belt |
Professor | Email | Website | Medicine | (608) 263-2443 | The Davis lab is focused on pancreatic beta cell biology. We aim to understand how beta-cells adapt to nutritional and metabolic stressors in the setting of obesity and insulin resistance. The long term goal is to identify beneficial adaptations that can be harnessed to develop new therapeutic strategies for the prevention and treatment of diabetes. Dr. Davis also participates in collaborative clinical research to move bench science to human studies that can inform us on novel approaches to obesity and diabetes. |
Gasch, Audrey |
Professor | Email | Website | Genetics | (608) 265-0859 | Our lab combines functional omics, computational modeling, and molecular dissection to present a systems-wide view of how eukaryotic cells respond to environmental stress. Cellular response to acute stress often spans many levels of physiology and includes coordinated changes in transcription, translation, post-translational protein modification, proliferation, and metabolism. We are particularly interested in how cells reroute metabolic flux to support defense strategies, and how altered flux is regulated in response to stress and toxins. |
Blum, Barak |
Assistant Professor | Email | Website | Cell and Regenerative Biology | (608) 265-5211 | Our research focuses on four main topics: |
Greenspan, Daniel S |
Professor | Email | Website | Cell and Regenerative Biology | (608) 262-4676 | We have studied extracellular matrix components, growth factors, and metalloproteinases involved in controlling cell |
Ney, Denise M |
Professor | Email | Website | Nutritional Sciences | (608) 262-4386 | Dr. Ney uses preclinical mouse models and human subjects to investigate novel approaches to the management of PKU and obesity. The nutritional variable is a protein isolated from cheese whey, glycomacropeptide (GMP); GMP is a 64-amino acid glycophosphopeptide that does not contain aromatic amino acids. GMP is a prebiotic with beneficial effects on the intestinal microbiota, bone health and inflammation. It promotes satiety in humans. Studies are being conducted to investigate calcium homeostasis and the pathophysiology of skeletal fragility in human PKU and to determine the temporal relationships between changes in body composition and the intestinal microbiota. |
Pike, J. Wesley |
Emeritus Professor | Email | Website | Biochemistry | (608) 262-8229 | Vitamin D, FGF23 and PTH represent endocrine hormones involved in the regulation of calcium and phosphate |
Roopra, Avtar |
Associate Professor | Email | Website | Neuroscience | (608) 265-9072 | Our early work demonstrated that REST is itself controlled by energy metabolism. Thus we were able to regulate a |
White, Heather |
Professor | Email | Website | Animal and Dairy Sciences | (608) 263-7786 | The transition to lactation period in dairy cattle represents the most critical time period for health and productivity. Dr. |
Kreeger, Pamela |
Professor | Email | Website | Biomedical Engineering | (608) 890-2915 | We are interested in multi-cellular interactions, with a focus on interactions between tumor and stromal cells in ovarian cancer. My lab uses a combination of engineered systems to study these interactions in vitro, experimental techniques, and computational models. |
Ntambi, James M. |
Professor | Email | Website | Biochemistry; Nutritional Sciences | (608) 265-3700 | The general theme of my research is to understand the genetic regulation of metabolism, adipocyte biology and differentiation. I am specifically interested in lipid metabolism and the genetic basis of obesity, cardiovascular disease, insulin resistance and diabetes and how dietary factors, hormones and environmental factors influence these disease states. In particular I study the regulation of the mammalian stearoyl-CoA desaturase (SCD) genes that encode an enzyme involved in the biosynthesis of monounsaturated fatty acids. We are using these genes as a model to understand nutrient gene interactions. |
Sauer, John-Demian (JD) |
Associate Professor | Email | Website | Medical Microbiology and Immunology | (608) 263-1529 | Our lab studies host pathogen interactions using Listeria monocytogenes as a model pathogen. L. monocytogenes is an |
Schoeller, Dale |
Professor | Email | Website | Nutritional Sciences | (608) 262-1082 | Our research focuses on energy metabolism with emphasis on human energy balance. We have expertise in stable isotope methods for energy expenditure using doubly labeled water and respiratory gas exchange; energy intake, substrate utilization using breath tests; and body composition using isotope dilution . We have studied environmental and behavior factors in the regulation of energy balance in obesity and wasting in clinical trials, population interventions and animal models. We also applied these methods to the validation of wearable monitors, survey tools and metabolic biomarkers. Human trials centered on the etiology, treatment, and prevention of obesity. |
Skala, Melissa |
Professor | Email | Website | Biomedical Engineering; Morgridge Institute for Research | (608) 316-4108 | We have developed label-free optical microscopy techniques to monitor metabolism in living samples on a single-cell level. Specifically, the auto-fluorescence of the metabolic co-enzymes NADH and FAD are imaged with two-photon microscopy in primary patient samples maintained in 3D organoids, and in mouse models in vivo. Our goal is to develop personalized screens for cancer treatment, and to understand interactions between the tumor cells and the tumor micro-environment. These microscopy tools are also used to study metabolic heterogeneity within tumor cells, immune cells, and fibroblasts, to guide the development of more effective cancer therapies. |
Thomas, Michael G |
Professor | Email | Website | Bacteriology | (608) 263-9075 | My laboratory focuses on discoverying, deciphering, and directing natural product biosynthesis by bacteria. We use genome mining and metagenomics to discover new natural product biosynthesis potential, with a particular emphasis on nonribosomal peptides and polyketides. We use biochemical, genetic, structural biology, and molecular techniques to decipher how these molecules are assembled. We use directed evolution and chimeric enzyme construction to direct natural product biosynthesis to generate 'unnatural' derivatives of these metabolites. The combination of these areas of emphasis are targeting the development of the next generation of drugs. |
Watters, Jyoti |
Professor | Email | Website | Comparative Biosciences | (608) 262-1016 | We are interested in understanding how paradigms of intermittent hypoxia (that mimic sleep apnea) cause epigenetic changes in central nervous system resident immune cells (microglia). In particular, we are investigating the roles of histone methylation and microRNAs in microglial inflammatory gene responses. Key microglial receptors and signaling pathways that are activated in response to intermittent hypoxia are a major focus. Since the prevalence of sleep apnea is increasing commensurate with the obesity epidemic, our studies also focus on how a high fat/high sugar diet influences the microglial immune response to intermittent hypoxia. |
Anderson, Rozalyn |
Professor | Email | Website | Medicine | (608) 256-1901 | My lab focuses on the role of metabolism in aging and age-related disease vulnerability. In addition to investigating the metabolism of normative aging in both mice and monkeys, we also study delayed aging by the dietary intervention of caloric restriction (CR), and use cell culture models to identify of processes and factors involved in the mechanisms of “anti-aging” by CR. Our studies suggest that age-associated changes in metabolic capacity are not just a biomarker of aging but may also be causally involved in creating increased disease vulnerability. The central hypothesis emerging from my work is that CR induces an active response involving a reprogramming of metabolism. A major current effort focuses on integrating transcriptional, proteomic, and metabolomic responses to CR, identifying differences in the response among tissues, and establishing how CR-induced changes metabolism intersect with delayed aging and increased resilience to age-related disease. |
Cantor, Jason |
Assistant Professor; Investigator | Email | Website | Biochemistry; Morgridge Institute for Research | (608) 316-4565 | Our lab has broad interests in modeling, understanding, and exploiting the impact of environmental factors on human cell metabolism, with a particular focus on hematological cancers and normal lymphocytes. We use an interdisciplinary approach to discover unforeseen biological (and pharmacological) phenomena in cell metabolism, cancer biology, and immunology that may have been overlooked or misinterpreted owing to the use of conventional in vitro (and perhaps even in vivo) model systems that poorly mimic physiologic conditions. Ultimately, we aim to translate our findings into new therapeutic opportunities. |
Simcox, Judith |
Assistant Professor | Email | Website | Biochemistry | (608) 262-8588 | The Simcox lab studies the regulation of thermogenic pathways by inter-organ lipid signaling. We use cold exposure as an energetic stress to functionally characterize lipid processing pathways that are important in the development and clearance of hepatic steatosis, aberrant lipid mobilization in Maturity onset diabetes of the young type 1 (MODY1), and inborn errors of metabolism. |